E. Coli Plasmid Vectors

E. Coli Plasmid Vectors

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The authors present a comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E. coli. The authors describe proven methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, 1 vectors, and phagemids. Common downstream applications such as mutagenesis of plasmids and the use of reporter genes, are also described.Sambrook, J. and Russell, D. W. (2001) Molecular Cloning: A Laboratory Manual, 3rd ed., Cold Spring Harbor Laboratory, Cold ... plasmid purification for capillary sequencing. Genome Res. 11, 1269a€“1274. 6. Engelstein, M., Aldredge, T. J., Madan, D., et al. ... Beckman Coulter Inc., www.beckman.com/products/pr2.asp.

Title:E. Coli Plasmid Vectors
Author: Nicola Casali, Andrew Preston
Publisher:Springer Science & Business Media - 2003-01-01

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